Recombinant Human Interleukin-12

英文名:
Cas号:
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产品参数
性状 Recombinant Human Interleukin-12 (HEK293-expressed) 是一种 HEK293 细胞来源的促炎细胞因子。
Synonyms
rHuIL-12; NKSF; CTL maturation factor (TCMF); Cytotoxic lymphocyte maturation factor (CLMF)
Species
Human

Source
HEK 293

Accession
P29459

Gene ID
3592

Molecular Weight
Approximately 75 kDa

AA Sequence
p35 Subunit:
RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS
p40 Subunit:
I

Biological Activity
The ED50 is <2 ng/mL as measured by NK cells, corresponding to a specific activity of >5 ×105 units/mg.

Appearance
Lyophilized powder.

Formulation
Lyophilized after extensive dialysis against PBS.

Endotoxin Level
<0.2 EU/μg, determined by LAL method.

Reconstitution
Reconstitute the lyophilized recombinant Human Interleukin-12 (HEK293-expressed) (rHuIL-12) to 100 µg/mL using ddH2O or diluted with PBS.

Storage & Stability
Lyophilized recombinant Human Interleukin-12 (HEK293-expressed) (rHuIL-12) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping
Room temperature in continental US; may vary elsewhere.

Background
Recombinant human IL-12 (hIL-12) is from the serum-free supernatants of stable IL-12-transduced HEK293 cells. Interleukin-12 is a heterodimeric, pro-inflammatory cytokine that is a key driver of cell-mediated immunity. Interleukin-12 (IL-12), previously known as natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric cytokine comprised of p40 and p35 subunits linked by an inter-subunit disulfide bridge. Among its many pleiotropic functions, IL-12 has been shown to induce: (i) IFNγ production; (ii) proliferation of activated T and NK cells; and (iii) TH1 differentiation. These functions are mediated by binding of intact IL-12 to its heterodimeric transmembrane receptor complex consisting of two chains, IL-12Rβ1 and IL-12Rβ2[1].
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