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Recombinant Human Stem Cell Factor
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性状 | Recombinant Human Stem Cell Factor (P.pastoris-expressed),是 c-kit 配体,是一种含二硫键的细胞因子,用于调节造血细胞和其他细胞如肥大细胞,生殖细胞的发育和功能。
Synonyms rHuSCF; Hematopoietic growth factor KL; MGF; Mast Cell Growth Factor Species Human Source P. pastoris Accession P21583 Gene ID 4254 Molecular Weight Approximately 20 kDa AA Sequence MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA Biological Activity The ED50 is <2 ng/mL as measured by human TF-1 cells, corresponding to a specific activity of 5 × 105 units/mg. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against 10 mM acetic acid. Endotoxin Level <1 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Human Stem Cell Factor (P.pastoris-expressed) (rHuSCF) to 100 µg/mL using ddH2O. Storage & Stability Lyophilized recombinant Human Stem Cell Factor (P.pastoris-expressed) (rHuSCF) is stored at -20°C. After reconstitution, it is stable at 4°C for 4 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background The recombinant human stem cell factor (rhSCF) protein specifically enhances the viability of human myeloid leukemia cell line TF-1 and the proliferation and maturation of human mast cell line LAD2 cell. Recombinant human SCF (rhSCF) can maintain hematopoietic stem cells and mast cell in culture, and it is widely tested in research into differentiation from embryonic stem cell (ESC)/induced pluripotent stem cell (iPSC)[1]. |
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