【产品介绍】Transcriptional Regulator ERG belongs to the
ETS family that is involved in the regulation of a
wide variety of cellular and developmental processes
(1). This family of transcription factors
contains approximately 30 members that share a
highly conserved DNA-binding domain (ETS domain) (2, 3)
and differs from each other in other domains
(such as absence or presence of the Pointed/SAM domain)
and are thus distinguished in sub-families (1). ERG
belongs to the Erg/Fli-1 sub-family. It has been
widely studied for its involvement in human
cancers; TMPRSS2-ERG fusion, which occurs on account
of translocations and interstitial deletions, is
implicated in aggressive forms of prostate cancer
(4, 5). ERG is linked to normal processes such as
mesoderm formation and is found to form functional
complexes with Jun/Fos, with the resulting ternary
complexes regulating expression of genes such
as metalloprotease-1 (MMP-1) and MMP-3 (1). It may
also participate in transcriptional regulation through
the recruitment of SETDB1 histone methyltransferase
and subsequent modification of local chromatin structure (2).
Recommended Applications
WB, IHC, ICC, IP
Applications and Recommended Dilution Factors WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:10 - 100
Species Reactivity * Human *Species cross-reactivity is based on WB analysis. Products Data
Western blot analysis on (A) Jurkat, (B) 293T, and (C)
MCF-7 cell lysates using anti-ERG RabMAb (cat. #5115-1). Western blot analysis on (A) 10 ng of Fli1
recombinant protein and (B) 10 ng of ERG
recombinant protein lysates using anti-ERG RabMAb
(cat. #5115-1). Immunohistochemical analysis of paraffin-embedded
human prostatic adenocarcinoma tissue using anti-ERG
RabMAb (cat. #5115-1). Specificity A synthetic peptide corresponding to residues on
the C-terminus in human ERG was used as an immunogen.
This antibody does not cross-react with Fli1. |
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