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Recombinant Human Interleukin-1 alpha

英文名：
Cas号：
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别 名
Cas号
M D L
分子式
分子量
产品参数
性状 Recombinant Human Interleukin-1 alpha 是一种多肽细胞因子，在炎症和免疫反应中起关键作用。

Synonyms
rHuIL-1α; Hematopoietin-1; IL1A; IL1F1; IL-1 alpha ; 重组人白细胞介素-1α
Species
Human

Source
E. coli

Accession
P01583

Gene ID
3552

Molecular Weight
Approximately 18.0 kDa

AA Sequence
SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA

Biological Activity
The ED50 is <1.0 pg/mL as measured by murine D10S cells, corresponding to a specific activity of >1.0 × 109 units/mg.

Appearance
Lyophilized powder.

Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4.

Endotoxin Level
<1 EU/μg, determined by LAL method.

Reconstitution
Reconstitute the lyophilized recombinant Human Interleukin-1 alpha (rHuIL-1α) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1 % BSA.

Storage & Stability
Lyophilized recombinant Human Interleukin-1 alpha (rHuIL-1α) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping
Room temperature in continental US; may vary elsewhere.

Background
Recombinant Human Interleukin-1 alpha is a non-glycosylated, polypeptide chain containing 159 amino acids and having a molecular mass of 18022 Dalton. The purified human monocyte derived IL-1 (mIL-1), a mixture of the two IL-1 forms but predominantly consisting of IL-1 beta, modulates eosinophil oxidative metabolism and enzyme secretion. Although the two major species of IL-1 (IL-1 alpha and IL-1 beta) have identical specific activities on T cells, the selective effects of human recombinant IL-1 (hrIL-1) alpha and hrIL-1 beta are reported on eosinophil function. Recombinant Human Interleukin-1 beta (hrIL-1 beta) causes a significant increase in arylsulfatase secretion (235.4±29% of resting secretion, P≤0.01) and beta-glucuronidase secretion (135.8±9.6% of resting secretion, P≤0.02) similar to mIL-1, Recombinant Human Interleukin-1 alpha (hrIL-1 alpha) has no effect on enzyme secretion. A mixture of hrIL-1 alpha and hrIL-1 beta reproduces the ability of mIL-1 to inhibit the oxidative response to suboptimal doses of phorbol myristate acetate (PMA). When eosinophils are separated into subpopulations by density gradients, eosinophil responses to IL-1 differ among the populations[1]. Members of the IL-1 family such as IL-1α and IL-1β are pro-inflammatory cytokines. Increased IL-1α and IL-1β levels have been detected separately in cigarette smoke (CS)-treated animals and inhibition of the respective signaling pathways result in anti-inflammatory activity[2].
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