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Recombinant Rat Ciliary Neurotrophic Factor
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| 性状 | Recombinant Rat Ciliary Neurotrophic Factor是一种多能性神经营养因子，可保护视网膜锥细胞和视杆细胞的光感受器。
Synonyms rRtCNTF; Ciliary Neurotrophic Factor Species Rat Source E. coli Accession P20294 Gene ID 25707 Molecular Weight Approximately 22.9 kDa AA Sequence AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM Biological Activity The ED50 is <30 ng/mL as measured by its ability to induce alkaline phosphatase production by TF-1 cells. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0. Endotoxin Level <0.2 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Rat Ciliary Neurotrophic Factor (rRtCNTF) to 100 μg/mL using ddH2O. Storage & Stability Lyophilized recombinant Rat Ciliary Neurotrophic Factor (rRtCNTF) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background Ciliary Neurotrophic Factor (CNTF) is an extracellular signaling protein in the neuroretinal and the interphotoreceptor matrix, which is associated with the membranes of the RPE, Muller and photoreceptor cells[1]. CNTF has neuroprotective effects on a variety of central and also peripheral nervous system neurons. Because it promotes differentiation and maturation of oligodendrocyte precursor cells to oligodendrocytes under in vitro conditions and thus improves remyelination. Importantly, it also increases the survival of mature oligodendrocytes[2]. |
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