Recombinant Mouse DKK-1 (CHO-expressed)

英文名:
Cas号:
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别 名
Cas号
M D L
分子式
分子量
产品参数
性状 Recombinant Mouse DKK-1 (CHO-expressed) 是 Wnt信号通路的有效抑制剂,是dickkopf 家族成员之一。
Synonyms
rMuDKK-1; mDkk-1; Dickkopf-1 ; 重组小鼠 DKK-1 (CHO细胞表达)
Species
Mouse

Source
CHO

Accession
O54908

Gene ID
13380

Molecular Weight
19-20 kDa

AA Sequence
SATLNSVLIN SNAIKNLPPP LGGAGGQPGS AVSVAPGVLY EGGNKYQTLD NYQPYPCAED EECGSDEYCS SPSRGAAGVG GVQICLACRK RRKRCMRHAM CCPGNYCKNG ICMPSDHSHF PRGEIEESII ENLGNDHNAA AGDGYPRRTT LTSKIYHTKG QEGSVCLRSS DCAAGLCCAR HFWSKICKPV LKEGQVCTKH KRKGSHGLEI FQRCYCGEGL ACRIQKDHHQ AS

Biological Activity
The ED50 is <6 μg/mL as measured in stimulation of alkaline phosphatase activity using CCl-226 cells.

Appearance
Lyophilized powder.

Formulation
Lyophilized after extensive dialysis against PBS.

Endotoxin Level
<0.2 EU/μg, determined by LAL method.

Reconstitution
Reconstitute the lyophilized recombinant Mouse DKK-1 (CHO-expressed) (rMuDKK-1) to 100 μg/mL using ddH2O or PBS.

Storage & Stability
Lyophilized recombinant Mouse DKK-1 (CHO-expressed) (rMuDKK-1) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping
Room temperature in continental US; may vary elsewhere.

Background
Mature mouse Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identity with human, rat, rabbit and bovine Dkk-1, respectively. It also shares 41% and 36% aa identity with human Dkk-2 and Dkk-4, respectively[1]. Dkk1 is a secreted Wnt inhibitor and member of a distinct multigene family, this inhibition plays a key role in heart, head and forelimb development during anterior morphogenesis of the embryo[2][3].
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