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Recombinant Mouse MCP-5/CCL12 (CHO-expressed)

英文名：
Cas号：
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别 名
Cas号
M D L
分子式
分子量
产品参数
性状 Recombinant Mouse MCP-5/CCL12 (CHO-expressed) 是一种有效的单核细胞活性趋化因子，参与过敏性炎症和宿主对病原体的反应。
Synonyms
rMuMCP-5/CCL12; C-C motif chemokine 12; MCP5; SCYA12 ; 重组小鼠MCP-5/CCL12 (CHO表达)
Species
Mouse

Source
CHO

Accession
Q62401

Gene ID
20293

Molecular Weight
9.3-11 kDa

AA Sequence
GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG

Biological Activity
The ED50 is <0.5 μg/mL as measured by CHO-K1/Gα15/mCCR2 cells (human Gα15 and mouse CCR2 stably expressed in CHO-K1 cells).

Appearance
Lyophilized powder.

Formulation
Lyophilized after extensive dialysis against PBS.

Endotoxin Level
<0.2 EU/μg, determined by LAL method.

Reconstitution
Reconstitute the lyophilized recombinant Mouse MCP-5/CCL12 (CHO-expressed) (rMuMCP-5/CCL12) to 100 μg/mL using ddH2O or PBS.

Storage & Stability
Lyophilized recombinant Mouse MCP-5/CCL12 (CHO-expressed) (rMuMCP-5/CCL12) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping
Room temperature in continental US; may vary elsewhere.

Background
MCP-5 mapped to the CC chemokine cluster on mouse chromosome 11 and was most closely related to human MCP-1 in structure (66% amino acid identity). Purified recombinant MCP-5 protein was a potent chemoattractant for peripheral blood monocytes, was only weakly active on eosinophils at high doses, and was inactive on neutrophils. MCP-5 induced a calcium flux in peripheral blood mononuclear cells, but not in purified murine eosinophils or neutrophils[1].
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