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| 生化科研试剂 >> 抗体产品 >> 一抗 |
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FAK Phospho (pY576/577) (PTK2)
Rabbit Monoclonal Antibody |
| 别 名： |
PTK2 antibody, FADK antibody, FAK antibody, FAK1 antibody, FRNK antibody, pp125FAK antibody, Focal adhesion kinase 1 antibody, Protein-tyrosine kinase 2 antibody, pp125FAK antibody |
| 货号 |
品名 |
规格 |
包装 |
单价 |
货期 |
库存 |
| 1227177781 |
FAK Phospho (pY576/577) (PTK2) |
|
100ul |
询价 |
现货 |
3天 |
| 提示信息：偏远地区如新疆西藏宁夏甘肃等地可能无法运送液体，下单前请联系客服。 |
| 性 状： |
【反应特异性】Human , Mouse
【克隆 ID】EP1832Y
【用途】WB , ICC , FC , IP
【抗原分子量】125kDa |
| 质量标准： |
【产品介绍】Focal adhesion kinase (FAK) is a
non-receptor protein-tyrosine kinase implicated in
signaling pathways involved in cell motility,
proliferation and apoptosis (1). FAK is composed of
a central catalytic domain flanked by large N-
and C-terminal regions. FAK is activated by
phosphorylation at tyrosine 397 in response to
integrin clustering which can be induced by cell adhesion
or antibody cross-linking or via G-protein-coupled
receptor (GPCR) occupancy by ligands such as bombesin
or lysophosphatidic acid (2-3). Phosphorylation of
FAK Tyr-397 creates a binding site for Src-family
kinases, and phosphorylation of FAK Tyr-576/Tyr-577 in
the kinase domain activation loop enhances
catalytic activity (4). Increased FAK expression has
been correlated with the enhanced motility and
invasiveness of human tumor cells, as well as with
promoting increased cell proliferation (5).
Recommended Applications
WB, ICC, IP, FC
Applications and Recommended Dilution Factors WB: 1:10,000 - 50,000
ICC: 1:50 - 100
IP: 1:40
FC: 1:20
Species Reactivity * Human, Mouse *Cross reactivity determined by western blot only. Products Data A. Western blot analysis on NIH-3T3 cell lysates
using anti-Phospho-FAK1 (pY576/577) RabMAb
(cat. #2183-1), 1:50.000 dilution. Cells were
either (A) untreated (B) treated with pervanadate. Specificity A phospho-specific peptide corresponding to
residues surrounding Tyrosine 576/577 of human FAK was
used as an immunogen. This antibody detects
FAK phosphorylated at Y576/577. |
| 贮 存： |
Storage Condition and Buffer Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt. |
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