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Alpha-Actinin 2

英文名：Alpha-Actinin 2
Cas号：
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别 名 Alpha-actinin skeletal muscle isof o r m 2, F-actin cross-linking protein
Cas号
M D L
分子式
分子量
产品参数 【产品介绍】Alpha-actinin is a ubiquitously
expressed protein found in numerous actin structures.
It consists of an N-terminal actin binding domain, a
central rod domain, a n d a C-terminal domain
a n d functions as a homodimer to cross-link
actin filaments. The rod domain determines the
distance between cross-linked actin filaments a n d
also serves as an interaction site f o r
several cytoskeletal a n d signaling proteins (1).
Alpha-actinins are a multigene family of four
actin-binding proteins related to dystrophin. The
two skeletal muscle isof o r ms of alpha-actinin
(ACTN2 a n d ACTN3) are maj o r structural components of
the Z-line involved in anch o r ing the
actin-containing thin filaments. In humans, ACTN2
is expressed in all muscle fibers, while ACTN3 expression
is restricted to a subset of type 2 fibers (2).

Recommended Applications
WB, IHC, FC, IP
Applications and Recommended Dilution Factors
WB: 1:200,000-1,000,000
IHC: 1:100-250
FC: 1:100
IP: 1:40
Species Reactivity *
Human, Mouse, Rat
* *Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on (A) Human skeletal muscle
(B) rat heart (C) human heart lysate using
anti-alpha-actinin 2 RabMAb (cat. # 2310-1).

B. Immunohistochemical analysis of paraffin-embedded
human muscles using anti-Alpha-Actinin 2 RabMAb
(cat. #2310-1).

Specificity
A synthetic peptide corresponding to residues on
the N-terminus of human Alpha-Actinin 2 was used
as an immunogen.
性状 【产品类型】Rabbit Monoclonal IgG
【反应特异性】Human , Mouse , Rat
【克隆 ID】EP2529Y
【用途】WB , IHC , FC , IP
【产品性状】
【阳性对照】
【产品浓度】
【细胞定位】
【抗原分子量】100 kDa
贮存 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
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