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Recombinant Human Interleukin-2
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| 性状 | Recombinant Human Interleukin-2 来源于E. coli，可用作治疗结核病 (TB) 的辅助免疫治疗剂。
Synonyms rHuIL-2; IL2; T-cell Growth Factor; TCGF; Aldesleukin Species Human Source E. coli Accession P60568 Gene ID 3558 Molecular Weight Approximately 15,300 Da AA Sequence MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSIIS TLT Biological Activity The ED50 is <0.1 ng/mL as measured by CTLL-2 cells, corresponding to a specific activity of >1 × 107 units/mg. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against 50mM Phosphate buffer, pH7.5, 5% Mannitol buffer. Endotoxin Level <1 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Human Interleukin-2 (rHuIL-2) to 100 µg/mL using ddH2O. Storage & Stability Lyophilized recombinant Human Interleukin-2 (rHuIL-2) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background Recombinant human interleukin-2 (IL-2) expressed as Escherichia coli is isolated as insoluble aggregates of protein (inclusion bodies) after cell breakage. Interleukin 2 (IL-2), a cytokine secreted by activated T cells, promotes the differentiation and proliferation of lymphoid cells as well as enhances the cell-mediated immune response to infections. Therefore IL-2 has been used as an adjunctive immunotherapeutic agent to control some infectious diseases, such as leishmaniasis, leprosy, and HIV infection[1]. |
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