Recombinant Human Interleukin-4 (CHO-expressed)

英文名:
Cas号:
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别 名
Cas号
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分子式
分子量
产品参数
性状 Recombinant Human Interleukin-4 (CHO-expressed) 是一种 CHO 细胞来源的有效的淋巴细胞生长因子,刺激 B 细胞增殖分化,参与炎症反应。
Synonyms
rHuIL-4; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra
Species
Human

Source
CHO

Accession
P05112

Gene ID
3565

Molecular Weight
Approximately 15 kDa

AA Sequence
HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS

Biological Activity
The ED50 is <0.25 ng/mL as measured by TF-1 human erythroleukemic cells, corresponding to a specific activity of >4 × 106 units/mg.

Appearance
Lyophilized powder.

Formulation
Lyophilized after extensive dialysis against PBS.

Endotoxin Level
<0.2 EU/μg, determined by LAL method.

Reconstitution
Reconstitute the lyophilized recombinant Human Interleukin-4 (CHO-expressed) (rHuIL-4) to 100 µg/mL using ddH2O or diluted with PBS.

Storage & Stability
Lyophilized recombinant Human Interleukin-4 (CHO-expressed) (rHuIL-4) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping
Room temperature in continental US; may vary elsewhere.

Background
Interleukin-4 (IL-4) is a cytokine that plays an important role in regulating inflammation and immune responses. It induces the differentiation of naïve helper T cells to Th2 cells. This cytokine binds the IL-4 receptor that also binds another cytokine interleukin 13 (IL-13), which may explain the overlapping functions of IL-4 and IL-13. IL-4, originally designated as B-cell growth factor-1 (BSFl), is described in the supernatant of activated EL-4 thymoma cells as a factor that can co-stimulate B-cells activated with submitogenic concentrations of anti-IgM[1].
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