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Recombinant Rat Stem Cell Factor (P.pastoris-expressed)
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性状 | Recombinant Rat Stem Cell Factor (P.pastoris-expressed) 是一种造血因子,与它的配体 c-kit 结合,在造血、精子发生和黑素生成中起着重要的作用。
Synonyms rRtSCF; Hematopoietic growth factor KL; MGF; Mast Cell Growth Factor ; 重组大鼠干细胞因子 (P.pastoris 表达) Species Rat Source P. pastoris Accession P21581 Gene ID 60427 Molecular Weight Approximately 18.3 kDa AA Sequence QEICRNPVTD NVKDITKLVA NLPNDYMITL NYVAGMDVLP SHCWLRDMVT HLSVSLTTLL DKFSNISEGL SNYSIIDKLG KIVDDLVACM EENAPKNVKE SLKKPETRNF TPEEFFSIFN RSIDAFKDFM VASDTSDCVL SSTLGPEKDS RVSVTKPFML PPVA Biological Activity The ED50 is <25 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >4 × 104 units/mg. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against PBS. Endotoxin Level <0.2 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Rat Stem Cell Factor (P.pastoris-expressed) (rRtSCF) to 100 µg/mL using ddH2O. Storage & Stability Lyophilized recombinant Rat Stem Cell Factor (P.pastoris-expressed) (rRtSCF) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background Stem Cell Factor is a hematopoietic cytokine, triggered by binding to its ligand c-kit, and plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. Stem Cell Factor (SCF) is applied for hematopoietic stem cell mobilization, exvivo stem/progenitor cell expansion, gene therapy, and immunotherapy[1]. Stem Cell Factor regulates cell survival, proliferation, differentiation, and migration in divergent cell types[2]. |
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