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Recombinant Human Vascular Endothelial Growth Factor 165 (P.pastoris-expressed)
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| 性状 | Recombinant Human Vascular Endothelial Growth Factor 165 (P. pastoris) 是血管内皮生长因子A (VEGF-A) 的亚型。 VEGF是血管内皮细胞特异性的肝素结合生长因子，能够诱导血管生成。
Synonyms rHuVEGF165; VPF; Folliculostellate cell-derived growth factor; Glioma-derived endothelial cell mitogen Species Human Source P. pastoris Accession P15692 Gene ID 7422 Molecular Weight Approximately 38.2 kDa AA Sequence APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR Biological Activity The ED50 is 1-5 ng/mL as measured by HUVEC cells, corresponding to a specific activity of 2 × 105 - 1 × 106 units/mg. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0. Endotoxin Level <0.5 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Human Vascular Endothelial Growth Factor 165 (P.pastoris-expressed) (rHuVEGF165) to 100 µg/mL using ddH2O. Storage & Stability Lyophilized recombinant Human Vascular Endothelial Growth Factor 165 (P.pastoris-expressed) (rHuVEGF165) is stored at -20°C. After reconstitution, it is stable at 4°C for 4 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background Vascular Endothelial Growth Factor (VEGF) has multiple isoforms, created by alternative splicing or proteolytic cleavage, and characterized by different receptor-binding and matrix-binding properties. These isoforms are known to give rise to a spectrum of angiogenesis patterns marked by differences in branching, which has functional implications for tissues. VEGF-A is a key member of the VEGF family of cytokines, along with VEGF-B, -C, -D, and PlGF. VEGF-A mediates angiogenesis, the expansion of an existing vascular bed by sprouting of new blood vessels. The vegfa gene is translated into a number of splice isoforms, the most notable in humans being VEGF121, VEGF165, and VEGF189[1]. |
| 贮存 |
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