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Recombinant Rat Interleukin-5
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性状 | Recombinant Rat Interleukin-5 是一种 T 细胞衍生的细胞因子,刺激 B 细胞生长并增强免疫球蛋白分泌。
Synonyms rRtIL-5; EDF; BCDFII; TRF Species Rat Source E. coli Accession Q9R2C9 Gene ID 24497 Molecular Weight Approximately 26.0 kDa AA Sequence MEIPMSTVVK ETLIQLSTHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEILFQNL SLIKKYIDGQ KEKCGEERRK TRHFLDYLQE FLGVMSTEWA MEV Biological Activity The ED50 is <0.4 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >2.5 × 106 units/mg. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against 20 mM Tris, pH 8.5. Endotoxin Level <0.2 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Rat Interleukin-5 (rRtIL-5) to 100 µg/mL using ddH2O. Storage & Stability Lyophilized recombinant Rat Interleukin-5 (rRtIL-5) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background Interleukin-5 (IL-5) is a T-cell derived cytokine which stimulates eosinophil production and activation in human, mice and sheep. IL-5 is active as a growth factor for mouse but not human B cells. The role of IL-5 on ruminant B cells has not been clearly defined. By hybridisation with human IL-5 cDNA, the ovine IL-5 gene is isolated from a liver genomic library. The IL-5 cDNA is obtained by reverse-transcriptase PCR using primers designed from the 5' and 3' coding sequence derived from the ovine IL-5 gene. The sequences of the cDNA shows that there is 79% and 73% nucleotide homology with the human and mouse sequences. The ovine IL-5 cDNA encodes a protein of 132 amino acids and the level of amino acid homology with human and mouse IL-5 is 64% and 56%, respectively. Two cysteine residues are conserved in ovine, human and mouse IL-5. There are two potential N-linked glycoyslation sites in ovine IL-5[1]. |
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- Recombinant Rat Interferon-gamma
- Recombinant Rat Interleukin-1 alpha (CHO-expressed)
- Recombinant Rat Interleukin-1 beta
- Recombinant Rat Interleukin-10
- Recombinant Rat Interleukin-5
- Recombinant Rat Interleukin-6 (HEK293-expressed)
- Recombinant Rat Interleukin-7, His (CHO-expressed)
- Recombinant Rat Oncostatin-M
- Recombinant Rat Stem Cell Factor (P.pastoris-expressed)