Recombinant Mouse Sonic Hedgehog (CHO-expressed)

英文名:
Cas号:
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别 名
Cas号
M D L
分子式
分子量
产品参数
性状 Recombinant Mouse Sonic Hedgehog 是一种能够协调多种小脑发育成熟过程的形态发生因子。
Synonyms
rMuShh; HHG-1; ShhNC ; 重组鼠源音猬因子
Species
Mouse

Source
CHO

Accession
Q62226

Gene ID
20423

Molecular Weight
Approximately 20 kDa

AA Sequence
CGPGRGFGKR RHPKKLTPLA YKQFIPNVAE KTLGASGRYE GKITRNSERF KELTPNYNPD IIFKDEENTG ADRLMTQRCK DKLNALAISV MNQWPGVKLR VTEGWDEDGH HSEESLHYEG RAVDITTSDR DRSKYGMLAR LAVEAGFDWV YYESKAHIHC SVKAENSVAA KSGG

Biological Activity
The ED50 is <1 μg/mL as measured by CCL-226 cells, corresponding to a specific activity of >1.0 × 103 units/mg.

Appearance
Lyophilized powder.

Formulation
Lyophilized after extensive dialysis against PBS.

Endotoxin Level
<0.2 EU/μg, determined by LAL method.

Reconstitution
Reconstitute the lyophilized recombinant Mouse Sonic Hedgehog (CHO-expressed) (rMuShh) to 100 μg/mL using ddH2O or PBS.

Storage & Stability
Lyophilized recombinant Mouse Sonic Hedgehog (CHO-expressed) (rMuShh) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping
Room temperature in continental US; may vary elsewhere.

Background
Sonic hedgehog (Shh) is a morphogenic factor that actively orchestrates many aspects of cerebellar development and maturation[1]. Sonic hedgehog (Shh) plays a critical role in post-natal skeletal muscle regeneration. Sonic hedgehog (Shh) is a crucial morphogen that regulates epithelial-mesenchymal interactions during embryogenesis. In adults, the Shh pathway has been shown to be up-regulated following skeletal muscle and myocardium ischemia, suggesting that the embryonic Shh pathway can be recruited[2].
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