Recombinant Rhesus Macaque Tumor Necrosis Factor alpha

英文名:
Cas号:
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分子式
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产品参数
性状 Recombinant Rhesus Macaque Tumor Necrosis Factor alpha 是一种参与全身炎症反应的细胞因子,是构成急性时相反应的细胞因子之一。
Synonyms
rRhTNF-α; Cachectin; TNF-α; TNFA ; 重组恒河猴肿瘤坏死因子α
Species
Rhesus Macaque

Source
E. coli

Accession
P48094

Gene ID
715467

Molecular Weight
Approximately 17.4 kDa

AA Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL

Biological Activity
The ED50 is <60 pg/mL as measured by mouse L-929 cells, corresponding to a specific activity of >1.6 × 107 units/mg.

Appearance
Lyophilized powder.

Formulation
Lyophilized after extensive dialysis against PBS.

Endotoxin Level
<0.2 EU/μg, determined by LAL method.

Reconstitution
Reconstitute the lyophilized recombinant Rhesus Macaque Tumor Necrosis Factor alpha (rRhTNF-α) to 100 μg/mL using ddH2O.

Storage & Stability
Lyophilized recombinant Rhesus Macaque Tumor Necrosis Factor alpha (rRhTNF-α) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping
Room temperature in continental US; may vary elsewhere.

Background
TNF-α (Tumor Necrosis Factor alpha) is a cytokine involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. TNF-α induces a model of preeclampsia in pregnant baboons (Papio hamadryas)[1]. TNF-α suppreses GnRH pulse generator activity at least partially through PG, but not CRH, dependent mechanisms[2].
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