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【产品介绍】In eukaryotic cells, protein synthesis
is regulated in response to various environmental
stresses by phosphorylating the alpha subunit of
the eukaryotic initiation factor 2 (eIF2alpha).
Four different eIF2alpha kinases have been identified
in mammalian cells, the heme-regulated inhibitor (HRI),
the interferon-inducible RNA-dependent kinase (PKR) and
the endoplasmic reticulum-resident kinase (PERK) and
GCN2, which was previously characterized from
Saccharomyces cerevisiae, Drosophila melanogaster
and Neurospora crassa. GCN2 is the unique eIF2alpha
kinase present in all eukaryotes from yeast to
mammals (1). GCN2, a sensor of amino acid deficiency,
plays a key role in yeast and mammals in modulating
amino acid metabolism as part of adaptation to
nutrient deprivation. (2). Results demonstrate that PERK
and GCN2 function to cooperatively regulate
eIF2alpha phosphorylation and cyclin D1 translation
after unfolded protein response pathway activation (3).
Recommended Applications
WB, IHC, ICC, FC
Applications and Recommended Dilution Factors WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:250 - 500
FC: 1:100 - 500
Species Reactivity * Human *Species cross-reactivity is based on WB analysis. Products Data
Western blot analysis on (A) HeLa, (B) 293T, (C) Molt-4,
and (D) A549 cell lysates using anti-GCN2 RabMAb
(cat. #5417-1). Immunohistochemical analysis of paraffin-embedded
human kidney tissue using anti-GCN2 RabMAb (cat. #5417-1). Specificity A synthetic peptide corresponding to residues near
the N-terminus of human GCN2 was used as an immunogen. |
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