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Recombinant Mouse Beta Defensin-3
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性状 | Recombinant Mouse Beta Defensin-3 是一种可诱导的抗菌肽,具有光谱抗菌活性。
Synonyms rMuBD-3; DEFB-3; HBD3; Beta-defensin 103 ; 重组小鼠 β-防御素-3 Species Mouse Source E. coli Accession Q9WTL0 Gene ID 27358 Molecular Weight Approximately 4.6 kDa AA Sequence KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K Biological Activity The ED50 is <20 μg/mL as measured corresponding to a specific activity of >50 units/mg. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against 2× PBS, pH 7.4. Endotoxin Level <1 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Mouse Beta Defensin-3 (rMuBD-3) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1% BSA. Storage & Stability Lyophilized recombinant Mouse Beta Defensin-3 (rMuBD-3) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background Recombinant Mouse Beta Defensin-3 peptide, produced from a baculovirus expression system, shows antimicrobial activity against P. aeruginosa PAO1 (MIC of 8 μg/mL) and Escherichia coli D31 (MIC of 16 μg/mL) in a salt-dependent manner[1]. Mouse Beta Defensin-3 peptide exhibits broad-spectrum antimicrobial activity, this peptide may serve as an innate defense against microbial invasion at specific mucosal surfaces in the mouse[2]. |
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