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【产品介绍】Cytochrome c is located in the mitochondria
of all aerobic cells and is involved in the
electron transport system that functions in
oxidative phosphorylation. It accepts electrons
from cytochrome b and transfers them to cytochrome
oxidase. In the process, the iron of the heme group,
which is identical to that of hemoglobin and
myoglobin, shifts from the ferrous to the ferric
state (1). Recent progress in studies on apoptosis
has revealed that cytochrome c is a pro-apoptotic factor.
It is released from its places on the outer surface of
the inner mitochondrial membrane at early steps of
apoptosis and, combining with some cytosolic
proteins, activates conversion of the latent
apoptosis-promoting protease pro-caspase-9 to its
active form. Cytochrome c release can be initiated by
the pro-apoptotic protein Bax. This process is blocked
by the anti-apoptotic proteins Bcl-2 and Bcl-xL (2).
Also, differences in amino acid composition of cytochrome
c have also been used as markers of biochemical evolution.
Recommended Applications
WB, IHC, ICC, IP
Applications and Recommended Dilution Factors WB: 1:10,000 - 50,000
IHC: 1:250 - 500
ICC: 1:100 - 250
IP: 1:10 - 100
Species Reactivity * Human, Mouse, Rat *Cross reactivity determined by western blot only. Products Data
Western blot analysis on (A) Molt-4, (B) SH-SY5Y,
(C) human heart, (D) human kidney, and (E) human
spleen lysates using anti-Cytochrome C RabMAb
(cat. #3895-1). Immunohistochemical analysis of paraffin-embedded
human kidney tissue using anti-Cytochrome C RabMAb
(cat. #3895-1). Immunofluorescent staining of HeLa cells
using anti-Cytochrome C RabMAb (cat. #3895-1). Specificity A synthetic peptide corresponding to residues in
human Cytochrome C was used as an immunogen. |
| 贮 存： |
Storage Condition and Buffer Store at -20° C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt. |
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